Wound healing assay

A wound healing assay is a laboratory technique used to study cell migration and cell–cell interaction. This is also called a scratch assay because it is done by making a scratch on a cell monolayer and capturing images at regular intervals by time lapse microscope.[1] While it has been described as straightforward, the technique has been criticized because of inconsistencies in its application from one experiment to another.[2]

References

  1. ↑ Rodriguez, Luis G.; Wu, Xiaoyang; Guan, Jun-Lin (2005). Methods in Molecular Biology, Volume 294. Humana Press. pp. 23–29. Retrieved December 18, 2015.
  2. ↑ Jonkman, James E. N.; Cathcart, Judith A.; Xu, Feng; Bartolini, Miria E.; Amon, Jennifer E.; Stevens, Katarzyna M.; Colarusso, Pina (September 3, 2014). "An introduction to the wound healing assay using live-cell microscopy". Cell Adhesion & Migration. 8 (5): 440–451. doi:10.4161/cam.36224. ISSN 1933-6918. Retrieved December 18, 2015.
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