Tryptophan 7-halogenase
| Tryptophan 7-halogenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.14.19.9 | ||||||||
| CAS number | 198575-11-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Tryptophan 7-halogenase (EC 1.14.19.9, PrnA, RebH) is an enzyme with systematic name L-tryptophan:FADH2 oxidoreductase (7-halogenating).[1][2] This enzyme catalyses the following chemical reaction
- tryptophan + FADH2 + Cl− + O2 + H+ 7-chloro-L-tryptophan + FAD + 2 H2O
In Lechevalieria aerocolonigenes the enzyme catalyses the initial step in the biosynthesis of rebeccamycin.[3]
The enzyme can use bromide ions (Br−) in place of chloride (Cl−).[4]
References
- ↑ Dong, C.; Kotzsch, A.; Dorward, M.; van Pee, K.H.; Naismith, J.H. (2004). "Crystallization and X-ray diffraction of a halogenating enzyme, tryptophan 7-halogenase, from Pseudomonas fluorescens". Acta Crystallogr. D. 60 (Pt 8): 1438–1440. doi:10.1107/S0907444904012521. PMID 15272170.
- ↑ Dong, C; Flecks, S; Unversucht, S; Haupt, C; Van Pée, K. H.; Naismith, J. H. (2005). "Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination". Science. 309 (5744): 2216–9. doi:10.1126/science.1116510. PMC 3315827
. PMID 16195462. - ↑ Bitto, Eduard; Huang, Yu; Bingman, Craig A.; Singh, Shanteri; Thorson, Jon S.; Phillips, George N. (2007). "The structure of flavin-dependent tryptophan 7-halogenase RebH". Proteins: Structure, Function, and Bioinformatics. 70 (1): 289–293. doi:10.1002/prot.21627. ISSN 0887-3585. PMID 17876823.
- ↑ Yeh, E.; Garneau, S.; Walsh, C.T. (2005). "Robust in vitro activity of RebF and RebH, a two-component reductase/halogenase, generating 7-chlorotryptophan during rebeccamycin biosynthesis". Proc. Natl. Acad. Sci. USA. 102 (11): 3960–3965. doi:10.1073/pnas.0500755102. PMC 554827. PMID 15743914.
External links
- Tryptophan 7-halogenase at the US National Library of Medicine Medical Subject Headings (MeSH)
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