Terpentetriene synthase

Terpentetriene synthase (EC 4.2.3.36, Cyc2) is an enzyme with systematic name terpentedienyl-diphosphate diphosphate-lyase (terpentetriene-forming).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

terpentedienyl diphosphate

\rightleftharpoons

terpentetriene + diphosphate

This enzyme requires Mg²⁺ for maximal activity but can use Mn²⁺, Fe²⁺ or Co²⁺ to a lesser extent.

References

↑ Dairi, T.; Hamano, Y.; Kuzuyama, T.; Itoh, N.; Furihata, K.; Seto, H. (2001). "Eubacterial diterpene cyclase genes essential for production of the isoprenoid antibiotic terpentecin". J. Bacteriol. 183: 6085–6094. doi:10.1128/jb.183.20.6085-6094.2001. PMID 11567009.
↑ Hamano, Y.; Kuzuyama, T.; Itoh, N.; Furihata, K.; Seto, H.; Dairi, T. (2002). "Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin". J. Biol. Chem. 277: 37098–37104. doi:10.1074/jbc.m206382200. PMID 12138123.
↑ Eguchi, T.; Dekishima, Y.; Hamano, Y.; Dairi, T.; Seto, H.; Kakinuma, K. (2003). "A new approach for the investigation of isoprenoid biosynthesis featuring pathway switching, deuterium hyperlabeling, and ¹H NMR spectroscopy. The reaction mechanism of a novel streptomyces diterpene cyclase". J. Org. Chem. 68: 5433–5438. doi:10.1021/jo026728a. PMID 12839434.

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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