Orobanche aegyptiaca

Egyptian broomrape
Scientific classification
Kingdom:	Plantae
(unranked):	Angiosperms
(unranked):	Eudicots
(unranked):	Asterids
Order:	Lamiales
Family:	Orobanchaceae
Genus:	Orobanche
Species:	O. aegyptiaca
Binomial name
Orobanche aegyptiaca Pers.

Orobanche aegyptiaca, Egyptian broomrape, is a plant which is an obligate holoparasite from the family Orobanchaceae with a complex life cycle. This parasite is most common in the Middle East and has a wide host range including many economically important crops.

Selective control of Egyptian broomrape is extremely difficult because the close association between host crop and parasite limits the use of most mechanical and herbicidal approaches.

Disease Cycle

A single Egyptian broomrape plant is capable of producing hundreds of thousands of extremely small (0.15-0.5 mm long) seeds. These seeds, dispersed by the wind, animals, or by more artificial means such as farm machinery, survive in the soil and have the ability to remain viable in the soil for more than 15 years. When the seeds are dropped, they are dormant. They require after-ripening, or further ripening after the seed has fallen from the plant, which is completed by the time the dry season has ended. As the rainy season begins, the seeds imbibe water and move into a conditioning phase which lasts 1–3 weeks, depending on whether the temperature remains optimal, between 60 and 70 degrees Fahrenheit. At this point, the seeds only need a chemical signal from the roots of a host plant to germinate. The seeds can remain in this conditioned state for several months. If the seed never receives the chemical signal to germinate and dries out, it will return to its dormant state, ready to be re-conditioned when the next rainy season begins.^[1]

When a conditioned seed receives a chemical signal from a host plant, it germinates, forming a germ tube which grows toward the host. From this germ tube, it grows a haustorium. The haustorium is the part of the broomrape which attaches the parasite to the host and grows into the host’s vascular system. The parasite is then able to draw out water and nutrients from its host.^[2] At this point, O. aegyptiaca grows rapidly, developing above-ground flower stalks. The flowers develop between February through May.^[3] A medium-sized plant can produce more than 400 flowers, which each produce around 500 seeds. The complete life cycle of this parasite, if completed in one season, lasts 10 to 15 weeks.

Hosts and Symptoms

The host range for Egyptian broomrape is fairly wide, including many broadleaf vegetables, field crops, and some ornamentals. Tomato, potato, tobacco, eggplant, peppers, peas, carrot, celery, mustard, spinach, and chrysanthemum are among the susceptible plants. In areas such as southern Russia melons are also potential hosts. Some plant species have been reported as being hosts for O. aegyptiaca that, in reality, are not hosts. Because the physical attachment of the parasite occurs below ground, it is critical to check that the plant’s roots are connected to the weed.^[4]

The most obvious sign of O. aegyptiaca parasitism is the visible parasitic plant near the base of the plant being attacked. Egyptian broomrape is a very short plant with purple flowers and a purple to brown stem. Plants that have been parasitized by this weed will show evidence of water stress, leaf yellowing, and stunted growth.^[5]

Environment

Orobanche aegyptiaca is adapted to soils with a high pH, like those in the Middle East where it is native, and requires high temperatures for ideal germination and growth. Because O. aegyptiaca is an obligate parasite it is found only in association with the crops it attacks, especially irrigated crops.^[6]

Management

Three main types of control exist for Orobanche aegyptiaca: chemical, cultural, and biological.

Chemical control can be achieved via soil fumigation with methyl bromide. This method is effective yet is rarely used because only a shallow layer of the soil is affected, it is a costly treatment, and methyl bromide poses environmental concerns.^[7] Another form of chemical control is soil solarization which is a solar heating of the soil by placing clear polyethylene sheets over moist soil. This kills the majority of seeds that are viable and induces secondary dormancy in the rest.^[8] Lastly, sulfonylurea and imidazolinone herbicides have been shown in many studies to be an effective form of chemical control for O. aegyptiaca with the efficacy depending on the method of application, the species of crop, and the timing of the application.^[9] One study has shown application times between 14 and 42 days after planting to be ideal for controlling Egyptian broomrape.^[10]

Some forms of cultural control of Orobanche aegyptiaca include sanitation efforts to help prevent the movement of seeds, individual picking of weeds by hand, and avoidance by changing the sowing time or not growing a host plant. Reducing spread of infected soil via farm machinery, avoidance of grazing on infected plants, and staying away from the use of hay produced from "Orobanche"-infested plants are all methods to help prevent the movement of seeds. The individual picking of weeds by hand is very important as the plant is able continue living as only a stem and produce a flower that can spread seeds even while not connected to the host. Any avoidance strategy must be carried out with a plant that cannot be parasitized by "Orobanche aegyptiaca" and could take up to 15–20 years to complete, as this is how long the plant's seeds can remain viable in the soil. Also, because seeds can safely pass through an animal digestive system, it’s important to ensure manure is not contaminated. Another option for cultural control is the use of trap crops or catch crops. Trap crops promote the germination of O. aegyptiaca seeds but will not allow parasitism; these include flax, mung bean, maize, and sorghum. Catch crops allow parasitism but are destroyed before the parasitic plant flowers, so the broomrape seeds cannot be produced and dispersed. Finally, there has been some success with host plant resistance in sunflower, fafa bean, lentil, and tomato but it has been very limited. Resistant cultivars of sunflower were bred in Russia and Spain but quickly lost their host-plant resistance due to selection towards more aggressive biotypes of the plant that had adapted to the new cultivars.^[11] In the resistant sunflower cultivars, germination was still induced but the plant's germ tube never develops after penetration by the haustorium.^[12] Because has been shown that macromolecules are transferred from the host to the parasite, one method for resistance is the introduction of anti-microbial gene from the flesh fly into the host genome. This allows for its protein product to be transferred to the parasite and work as a means of control.^[13]

Most forms of biological control are still being developed and studied although studies have shown three species to be potential forms of control: Fusarium oxysporum, Fusarium solani, and Sclerotinia sclerotiorum. Fusarium oxysporum has been shown to successfully control Orobanche aegyptica in sunflower and tobacco while Sclerotinia sclerotiorum has been shown to cause wet rot in the plant without having any negative impact on the host. Field experiments carried out in Hungary showed promise for control with some "Fusarium solani" isolates. The "Fusarium" species usually work through phytotoxins that help them to conquer Orobanche aegyptica 's defenses and establish themselves.^[14]

Importance

Because Egyptian broomrape can cause severe damage to economic crops and ornamentals, it is important to understand the plant as a parasite and to fully comprehend its life cycle. By understanding its life cycle, management is possible.

In Israel, the production of processing tomatoes is in danger as a result of high crop yield losses due to heavy infestations of O. aegyptiaca. Approximately $1.3 to 2.6 billion have been lost in annual food crop losses in the regions near the Mediterranean, Northern Africa, and Asia.^[15] Egyptian broomrape and branched broomrape (O. ramosa) have infested an annual average of 2.6 million ha of crops from the Solanaceae family, including tomatoes, potatoes, and eggplant.^[16]

References

1. Control of Egyptian Broomrap (Orobanche aegyptiaca) and Other Weeds by Means of Solar Heating of the Soil by Polyethylene Mulching, R. Jacobsohn, A. Greenberger, J. Katan, M. Levi and H. Alon, Weed Science, Vol. 28, No. 3 (May, 1980), pp. 312–316
2. http://www.infonet-biovision.org/default/ct/705/pests
3. Effect of Egyptian Broomrape (Orobanche aegyptiaca) Seed-Burial Depth on Parasitism Dynamics and Chemical Control in Tomato, Hanan Eizenberg, Tal Lande, Gay Achdari, Asia Roichman and Joseph Hershenhorn, Weed Science, Vol. 55, No. 2 (Mar. - Apr., 2007), pp. 152–156
4. Application Soil Solarization, on the Control of Egyptian Broomrape in Greenhouse, Sedigheh S, Aptin R, Zoheir Y.A, International Journal of Natural and Engineering Sciences 3 (1): 59-64, 2009
5. Ghannam, I., R. Barakat, & M. Al-Masri. "Biological control of Egyptian broomrape (Orobanche aegyptiaca) using Fusarium spp.." Phytopathologia Mediterranea [Online], 46.2 (2007): 177-184. Web. 24 Oct. 2012
6. http://www.flowersinisrael.com/Orobancheaegyptiaca_page.htm
7. HANAN EIZENBERG, YAAKOV GOLDWASSER, SHMUEL GOLAN, DINA PLAKHINE, and JOSEPH HERSHENHORN (2004) Egyptian Broomrape (Orobanche aegyptiaca) Control in Tomato with Sulfonylurea Herbicides—Greenhouse Studies. Weed Technology: July 2004, Vol. 18, No. 3, pp. 490–496.
8. Eizenberg, H., D. Plakhine, J. Hershenhorn, Y. Kleifeld, and B. Rubin. "Resistance to Broomrape (Orobanche Spp.) in Sunflower (Helianthus Annuus L.) Is Temperature Dependent." Journal of Experimental Botany 54.385 (n.d.): 1305-311
9. Hamamouch, Noureddine. "Engineering Resistance to Orobanche Aegyptiaca: Evidence of Sarcotoxin IA as an Anti-Parasite Protein and Macromolecule Movement from Host to Parasite." Diss. Virginia Polytechnic Institute and State University, 2004