Hexadimethrine bromide
 | |
| Names | |
|---|---|
| IUPAC name
1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide | |
| Other names
Polybrene | |
| Identifiers | |
28728-55-4  | |
| ECHA InfoCard | 100.209.698 |
| Properties | |
| (C13H30Br2N2)n, linear form | |
| Molar mass | variable |
| Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
Hexadimethrine bromide (commercial brand name Polybrene) is a cationic polymer used to increase the efficiency of transduction of certain cells with virus in cell culture.[1] Hexadimethrine bromide acts by neutralizing the charge repulsion between virions and sialic acid on the cell surface.[2] Use of Polybrene can improve transduction efficiency 100-1000 fold[3] although it can be toxic to some cell types. Polybrene in combination with DMSO shock is used to transfect some cell types such as NINH-3T3 and CHO[3] . It has other uses, including a role in protein sequencing.
Historically, hexadimethrine bromide was sometimes used to reverse heparin anticoagulation during open-heart surgery. Its use has been supplanted by protamine sulfate.
References
Polybrene® is a registered trademark of Abbott Laboratories Corp.
- ↑ Howard e. Davis; Jeffery R. Morgan; Martin L. Yarmush (2002). "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on the target cell membranes.". Biophysical Chemistry. 97 (2): 159–172. doi:10.1016/S0301-4622(02)00057-1.
- ↑ Howard E. Davis; Matthew Rosinski; Jeffrey R. Morgan; Martin L. Yarmush; et al. (2004). "Charged Polymers Modulate Retrovirus Transduction via Membrane Charge Neutralization and Virus Aggregation.". Biophysical Journal. 86 (2): 1234–42. doi:10.1016/S0006-3495(04)74197-1. PMC 1303915
. PMID 14747357. - 1 2 "Polybrene Infection / Transfection Reagent | TR-1003-G". www.emdmillipore.com. Retrieved 2016-12-02.