Gellan lyase

Gellan lyase (EC 4.2.2.25) is an enzyme with systematic name gellan beta-D-glucopyranosyl-(1->4)-D-glucopyranosyluronate lyase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

Eliminative cleavage of beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyluronate bonds of gellan backbone releasing tetrasaccharides containing a 4-deoxy-4,5-unsaturated D-glucopyranosyluronic acid at the non-reducing end.

The tetrasaccharide produced from deacetylated gellan is beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp.

References

↑ Hashimoto, W.; Maesaka, K.; Sato, N.; Kimura, S.; Yamamoto, K.; Kumagai, H.; Murata, K. (1997). "Microbial system for polysaccharide depolymerization: enzymatic route for gellan depolymerization by Bacillus sp. GL1". Arch. Biochem. Biophys. 339: 17–23. doi:10.1006/abbi.1996.9851. PMID 9056228.
↑ Hashimoto, W.; Sato, N.; Kimura, S.; Murata, K. (1998). "Polysaccharide lyase: molecular cloning of gellan lyase gene and formation of the lyase from a huge precursor protein in Bacillus sp. GL1". Arch. Biochem. Biophys. 354: 31–39. doi:10.1006/abbi.1998.0674. PMID 9633595.
↑ Miyake, O.; Kobayashi, E.; Nankai, H.; Hashimoto, W.; Mikami, B.; Murata, K. (2004). "Posttranslational processing of polysaccharide lyase: maturation route for gellan lyase in Bacillus sp. GL1". Arch. Biochem. Biophys. 422: 211–220. doi:10.1016/j.abb.2003.12.015. PMID 14759609.

External links

Gellan lyase at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

This article is issued from Wikipedia - version of the 5/19/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.