Bile-acid 7alpha-dehydroxylase

Bile-acid 7alpha-dehydroxylase (EC 1.17.99.5, cholate 7alpha-dehydroxylase, 7alpha-dehydroxylase, bile acid 7-dehydroxylase) is an enzyme with systematic name deoxycholate:NAD⁺ oxidoreductase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

(1) deoxycholate + NAD⁺ + H₂O

\rightleftharpoons

cholate + NADH + H⁺

(2) lithocholate + NAD⁺ + H₂O

\rightleftharpoons

Bile-acid 7alpha-dehydroxylase is highly specific for bile acid substrates.

References

↑ White, B.A.; Cacciapuoti, A.F.; Fricke, R.J.; Whitehead, T.R.; Mosbach, E.H.; Hylemon, P.B. (1981). "Cofactor requirements for 7α-dehydroxylation of cholic and chenodeoxycholic acid in cell extracts of the intestinal anaerobic bacterium, Eubacterium species V.P.I. 12708". J. Lipid Res. 22 (6): 891–898. PMID 7276750.
↑ White, B.A.; Paone, D.A.; Cacciapuoti, A.F.; Fricke, R.J.; Mosbach, E.H.; Hylemon, P.B. (1983). "Regulation of bile acid 7-dehydroxylase activity by NAD⁺ and NADH in cell extracts of Eubacterium species V.P.I. 12708". J. Lipid Res. 24 (1): 20–27. PMID 6833878.
↑ Coleman, J.P.; White, W.B.; Hylemon, P.B. (1987). "Molecular cloning of bile acid 7-dehydroxylase from Eubacterium sp. strain VPI 12708". J. Bacteriol. 169 (4): 1516–1521. PMC 211977. PMID 3549693.
↑ Russell, D.W. (2003). "The enzymes, regulation, and genetics of bile acid synthesis". Annu. Rev. Biochem. 72: 137–174. doi:10.1146/annurev.biochem.72.121801.161712. PMID 12543708.

Other oxidoreductases (EC 1.15-1.21)

1.15: Acting on superoxide as acceptor	Superoxide dismutase SOD1 SOD2 SOD3

1.16: Oxidizing metal ions	Ceruloplasmin

1.17: Acting on CH or CH2 groups	Xanthine oxidase Ribonucleotide reductase 4-Hydroxy-3-methylbut-2-enyl diphosphate reductase

1.18: Acting on iron-sulfur proteins as donors	Nitrogenase

1.19: Acting on reduced flavodoxin as donor	Nitrogenase (flavodoxin)

1.20: Acting on phosphorus or arsenic in donors	Glutaredoxin GLRX GLRX2 GLRX3 GLRX5

1.21: Acting on X-H and Y-H to form an X-Y bond	Isopenicillin N synthase Columbamine oxidase Reticuline oxidase Sulochrin oxidase (+)-bisdechlorogeodin-forming (-)-bisdechlorogeodin-forming Aureusidin synthase Tetrahydrocannabinolic acid synthase Cannabidiolic acid synthase Dichlorochromopyrrolate synthase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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