Abietadienol hydroxylase

Abieta-7,13-dien-18-ol hydroxylase (EC 1.14.13.109, CYP720B1, PTAO) is an enzyme with systematic name abieta-7,13-dien-18-ol,NADPH:oxygen oxidoreductase (18-hydroxylating).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

abieta-7,13-dien-18-ol + NADPH + H⁺ + O₂

\rightleftharpoons

abieta-7,13-dien-18-al + NADP⁺ + 2 H₂O (overall reaction)

(1a) abieta-7,13-dien-18-ol + NADPH + H⁺ + O₂

\rightleftharpoons

abieta-7,13-dien-18,18-diol + + NADP⁺ + H₂O:

(1b) abieta-7,13-dien-18,18-diol

\rightleftharpoons

abieta-7,13-dien-18-al + H₂O (spontaneous)

Abietadienol hydroxylase is a heme-thiolate protein (P-450).

References

↑ Funk, C.; Croteau, R. (1994). "Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis". Arch. Biochem. Biophys. 308: 258–266. doi:10.1006/abbi.1994.1036. PMID 8311462.
↑ Funk, C.; Lewinsohn, E.; Vogel, B.S.; Steele, C.L.; Croteau, R. (1994). "Regulation of oleoresinosis in grand fir (Abies grandis) (coordinate induction of monoterpene and diterpene cyclases and two cytochrome P450-dependent diterpenoid hydroxylases by stem wounding)". Plant Physiol. 106 (3): 999–1005. doi:10.1104/pp.106.3.999. PMC 159624. PMID 12232380.
↑ Ro, D.K.; Arimura, G.; Lau, S.Y.; Piers, E.; Bohlmann, J. (2005). "Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase". Proc. Natl. Acad. Sci. USA. 102 (22): 8060–8065. doi:10.1073/pnas.0500825102. PMC 1138258. PMID 15911762.

Oxidoreductases: dioxygenases, including steroid hydroxylases (EC 1.14)

1.14.11: 2-oxoglutarate	Prolyl hydroxylase Lysyl hydroxylase

1.14.13: NADH or NADPH	Flavin-containing monooxygenase FMO1 FMO2 FMO3 FMO4 FMO5 Nitric oxide synthase NOS1 NOS2 NOS3 Cholesterol 7 alpha-hydroxylase Methane monooxygenase 3A4 Lanosterol 14 alpha-demethylase 24-hydroxycholesterol 7α-hydroxylase

1.14.14: reduced flavin or flavoprotein	19A1 2D6 2E1

1.14.15: reduced iron-sulfur protein	11B1 11B2 11A1

1.14.16: reduced pteridine (BH4 dependent)	Phenylalanine hydroxylase Tyrosine hydroxylase Tryptophan hydroxylase

1.14.17: reduced ascorbate	Dopamine beta-hydroxylase

1.14.18-19: other	Tyrosinase Stearoyl-CoA desaturase-1

1.14.99 - miscellaneous	Cyclooxygenase Heme oxygenase (HMOX1) Squalene monooxygenase 17A1 21A2

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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