4-hydroxybutanoyl-CoA dehydratase

4-hydroxybutanoyl-CoA dehydratase (EC 4.2.1.120) is an enzyme with systematic name 4-hydroxybutanoyl-CoA hydro-lyase.^[1]^[2]^[3]^[4]^[5] This enzyme catalyses the following chemical reaction

4-hydroxybutanoyl-CoA

\rightleftharpoons

but-3-enoyl-CoA + H₂O

This enzyme contains FAD and a [4Fe-4S] iron-sulfur cluster.

References

↑ Bartsch, R.G.; Barker, H.A. (1961). "A vinylacetyl isomerase from Clostridium kluyveri". Arch. Biochem. Biophys. 92: 122–132. doi:10.1016/0003-9861(61)90226-0. PMID 13687513.
↑ Scherf, U.; Sohling, B.; Gottschalk, G.; Linder, D.; Buckel, W. (1994). "Succinate-ethanol fermentation in Clostridium kluyveri: purification and characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA Δ³-Δ²-isomerase". Arch. Microbiol. 161 (3): 239–245. doi:10.1007/bf00248699. PMID 8161284.
↑ Scherf, U.; Buckel, W. (1993). "Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA Δ³-Δ²-isomerase from Clostridium aminobutyricum". Eur. J. Biochem. 215 (2): 421–429. doi:10.1111/j.1432-1033.1993.tb18049.x. PMID 8344309.
↑ Muh, U.; Cinkaya, I.; Albracht, S.P.; Buckel, W. (1996). "4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: characterization of FAD and iron-sulfur clusters involved in an overall non-redox reaction". Biochemistry. 35 (36): 11710–11718. doi:10.1021/bi9601363. PMID 8794752.
↑ Berg, I.A.; Kockelkorn, D.; Buckel, W.; Fuchs, G. (2007). "A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea". Science. 318: 1782–1786. doi:10.1126/science.1149976. PMID 18079405.

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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